Application Note – Bioaerosol N.6 – Sampling Plan
Introduction
The air sampling plan in check-list format aids both the operator’s supervisor and the operator in remembering all the points required for correct microbiological air sampling.
| 1. STAGE OF SAMPLING PLAN | ACTIONS AND COMMENTS |
| Date of sampling | |
| Reason for sampling | |
| Air Sampler Type | |
| Accessories | |
| table tripod | |
| floor tripod | |
| remote switch | |
| additional sterilized heads | |
| aerosol disinfectant | |
| Sampling form | |
| Sampling area | |
| Sampling location | |
| Operator’s name | |
| Sample file | |
| Time of sampling (0-24 hours) | |
| Sampling interval | |
| Volume of air | |
| Replicate sampling | |
| Sample identification number | |
| Type of plate | |
| Number of people during sampling | |
| Type of media | |
| Micro-organisms to be counted | |
| Micro-organisms to be identified | |
| HVAC switch on or off | |
| Temperature of environment | |
| indoor | |
| outdoor | |
| Atmospheric humidity | |
| In situ head sterilization |
| 2. CONTACT PLATES OPANING/RE PACKING | ACTIONS AND COMMENTS |
| Container used | |
| Container code | |
| Disinfectant | |
| Sterile gloves (clean room) |
| 3. TRANSPORT TO THE LABORATORY | ACTIONS AND COMMENTS |
| Address of laboratory | |
| Working hours of the laboratory | |
| Dead-line for sample transport | |
| Transport case | |
| Refrigerated case | |
| Method of transport | |
| Person responsible | |
| Means of transport |
| 4.SAMPLE PREPARATION IN LABORATORY | ACTIONS AND COMMENTS |
| Is immediate analysis needed? | |
| Which data are recorded and how? | |
| Incubation temperature | |
| Incubation time | |
| Sample report form details |
Reference
IES-RP-CC023.1-Microorganisms in clean rooms- Institute of Environmental Sciences
