BEST OF THE WEB V.13
CHEMISTRY
P. Gramatica. Introduction to the need of alternative methods in “REACH”. Science and Technology – La Chimica e l’Industria Dec. 2009. P 132-137.
Chemicals. Legislation. Processes. Technologies.
LIFE SCIENCE
Chemanager 1/2010. The Newspaper for the Chemical and Life Sciences markets. Celebrating the Human Footprint.
Nature Methods. Techniques for life science scientists and chemists. January 2010, Volume 7, N.1.
Contents: Reprogrammable mice. High-throughput SRM assay development. Imaging apoptosis in live cells. Quantifying signaling pathways. Methods of the year 2009.
Method of the year. iPS cells: potent stuff. Now that the generation of induced pluripotent stem cells is becoming routine, researchers can get on to the more exciting prospect of using the cells to make discoveries in disease and basic biology.
Nature Methods. Vol. 7. No.1. January 2010. Pages 17/21.
MEDICAL DEVICES
European Medical Device Technology – EMDT – January 2010. Contents: Biomaterials and real or imaginary infections. The challenge for biomaterials in urology.
LABORATORY
Inside Laboratory Management. AOAC International. Nov / Dec 2009.
GOOD LABORATORY PRACTICE
A. Fox. Technical records: How much information is sufficient?
Inside Laboratory Management – AOAC International – Nov / Dec 2009. Page9.
BIOTECHNOLOGY
The top 10 Bio Techniques journal articles that were read on-line in 2009, as measured by page views:Several reviews and novel methods were popular among readers at BioTechniques.com in 2009. Topics ranged from protein expression to stem cells to forensics. Here are the top 10 BioTechniques journal articles that were read online in 2009, as measured by page views:
1 - Analysis of insoluble proteins: Sarah Trimprin and Bill Brizzard present an overview of methods for the purification of expressed proteins using fusion tags and mass spectrometry.
2 - Twenty-five years of quantitative PCR for gene expression analysis: Researchers at Penn State publish a review focusing on the use of qPCR for quantification of RNA levels.
3 - Emerging molecular approaches in stem cell biology: This review discusses the current understanding of molecular similarities and differences among various stem cell types, including an overview of iPS cells and the current state of systems biology.
4 - Transcriptional effects of transfection: Researchers present the potential for misinterpretation of gene expression data.
5 - Cell-free cloning using multiply-primed rolling circle amplification with modified RNA primers: Japanese researchers devised a way to amplify and ligate a large construct without undesired DNA sequences and at microgram quantities within one day.
6 - Positive-selection vector for direct protein expression: Scientists in Germany and Switzerland developed high-level protein expression using a novel positive-selection vector and a T7 polymerase-based expression cassette.
7 - Subcellular protein extraction from human pancreatic cancer tissues: Researchers in Germany used a scheme of tissue-slicing and subsequent cell isolation to extract a variety of proteins from across a tumor.
8 - Assessing a novel room-temperature RNA storage medium for compatibility in microarray gene expression analysis: Hernandez, Mondala, and Head at the Scripps Research Institute’s DNA Array Core Facility evaluated a novel RNA storage method that uses anhydrobiosis to protect samples at room temperature.
9 - Thin-sheet laser imaging microscopy for optical sectioning of thick tissues: Scientists at the University of Minnesota, in collaboration with the Technische Universitat in Ilmenau, Germany have moved specimens from mice, rats, and zebrafish through a laser imaging microscope and stitched the image columns together for optimal resolution and focus.
10 - Extracting evidence from forensic DNA analyses: future molecular biology directions: Bruce Budowle and Angela van Daal have published a review identifying where molecular biology will likely guide the field of forensics.
Keywords: top 10 peer-reviewed BioTechniques journal 2009
