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Disinfectant efficacy methods

(A) AOAC – Association of Official Analytical Chemists test methods

TEST	PURPOSE
AOAC 6.2.02 Official Method 991.47	Disinfectants testing against Salmonella Choleraesuis Hard surface carrier Test Method
AOAC 6.2.03 Official Method 991.48	Disinfectants testing against Staphylococcus aureus Hard surface carrier Test Method
AOAC 6.2.05 Official Method 991.49	Disinfectants testing against Pseudomonas aeruginosa Hard surface carrier Test Method
AOAC 6.2.04 Official Method 955.15	Disinfectants testing against Staphylococcus aureus Use-Dilution Method
AOAC 6.2.01 Official Method 955.14	Disinfectants testing against Salmonella Choleraesuis Use-Dilution Method
AOAC 6.2.06 Official Method 994.02	Disinfectants testing against Pseudomonas aeruginosa Use-Dilution Method
AOAC 6.3.02 Official Method 955.17	Fungicidal acivity of disinfectants using Trichophyton mentagrophytes
AOAC 6.3.05 Official Method 966.04	Sporicidal activity of disinfectants
AOAC 6.3.06 Official Method 965.12	Tubercolocidal activity of disinfectants

(B) European Committee for Normalization TC216 Committee. Disinfectant Effectiveness Tests – European Tiered Test Approach

PHASE	TEST TYPE	PURPOSE
Phase 1	Quantitative suspension test under unsoiled conditions for basic activity	To establish that a product has bactericidal and/or fungicidal activity without regard to specific conditions of intended use. If the product passes, it qualifies for further testing
Phase 2 Step 1	Quantitative suspension test under conditions representative of practical use	To establish that a product has bactericidal, and/or fungicidal, and or sporicidal, and/or virucidal, and/or tubercolocidal, and so forth, activity under conditions appropriate to its intended use in laboratory conditions. If the product passes, it qualifies for generic disinfectancy claims.
Phase 2 Step 2	Quantitative surface test under conditions representative of practical use	Further laboratory test such as hand wash and hand rub tests, and tests on inert surface to establish that products have microbicidal activity against surface attached micro-organisms. If the product passes, it qualifies for disinfectancy claims on surfaces.
Phase 3	Field test under practical conditions	Confirmatory test is expected to confirm the findings of Phaeses 1 and 2.

(C) British Standards (BS) EN Test Methods

TEST	PURPOSE
BS EN 1276	Quantitative suspension test (QST) for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic, and institutional areas.
BS EN 1650	Quantitative suspension test (QST) for the evaluation of fungicidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic, and institutional areas.
BS EN 13704	Quantitative suspension test (QST) for the evaluation of sporicidal activity of chemical disinfectants used in food, industrial, domestic, and institutional areas.
BS EN 13697	Quantitative non porous surface test for the evaluation of bactericidal and/or fungicidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic, and institutional areas.