EXPERT – MICROBIOLOGY & GLP

Question:

Which are the protocols for the correct use of the different types of biological indicators according to GLP?

Answer:

• Population Assay – Metal discs, Metal strips, Wires 

• Population Assay – Prospore Ampoule 

• Population Assay – Spore strips, Discs, Thread, Metal 

• Population Assay – Spore suspension 

• Steam D-value Determination 

Notes

1. USP recommends using 3 specimen of the relevant biological indicator for determining a total viable spore count.

2. Perform population count on 3 separate biological indicators and average counts.

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